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svg p12 cells  (ATCC)
96
ATCC svg p12 cells
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Svg P12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fetal glial cells svg p12  (ATCC)
94
ATCC human fetal glial cells svg p12
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Human Fetal Glial Cells Svg P12, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human astrocyte cell line nha  (Procell Inc)
86
Procell Inc human astrocyte cell line nha
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Human Astrocyte Cell Line Nha, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glial cell line svg p12  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications glial cell line svg p12
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Glial Cell Line Svg P12, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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svg p12  (LGC Promochem)
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LGC Promochem svg p12
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Svg P12, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human astrocyte cell line svg p12  (Ubigene Biosciences Co Ltd)
86
Ubigene Biosciences Co Ltd human astrocyte cell line svg p12
Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in <t>SVG</t> <t>p12</t> cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.
Human Astrocyte Cell Line Svg P12, supplied by Ubigene Biosciences Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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svg p12 cells  (CLS Cell Lines Service GmbH)
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Image Search Results


Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in SVG p12 cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.

Journal: Cell death & disease

Article Title: Reduction of spermine synthase enhances autophagy to suppress Tau accumulation.

doi: 10.1038/s41419-024-06720-8

Figure Lengend Snippet: Fig. 4 SMS knockdown upregulates autophagy and suppresses Tau accumulation in human neuronal or glial cell lines. A Western blot of Tau, EGFP, autophagy marker LC3, cargo recruiter p62 and SMS in SH-SY5Y cells with Tau/EGFP plasmids and Control/SMS siRNA transfection. The image is a representative of four separate experiments. B Quantification of the protein levels of Tau (5A6), EGFP, LC3-I (cytoplasmic), LC3-II (autophagosome-associated), p62, and SMS in (A). All the protein levels were normalized with the β-Actin level. All the values were further normalized by that of the control cells. n = 4; Student’s t test (Tau or EGFP) or two-way ANOVA Sidak’s multiple comparisons (others). C p62 staining and Alexa 647-conjugated Tau K18 fibrils in SVG p12 cells with Control/SMS siRNA transfection. The images are representatives of five fields. D, E Quantification of the size (area) (D) and intensity (E) of the Alexa 647-conjugated Tau K18 fibrils in (C). n = 5; Student’s t test. Data represent mean ± SEM.

Article Snippet: Control or SMS siRNA are transfected into SVG p12 cells (CRL-8621, ATCC) on coverslips in 12-well plates as mentioned above.

Techniques: Knockdown, Western Blot, Marker, Control, Transfection, Staining